Research

Study outlines ‘perfect storm’ that led to Colombia’s antibiotic resistance epidemic


Cesar-Arias
By Hannah Rhodes | November 09, 2017

The nearly simultaneous emergence of a gene responsible for producing carbapenemases – enzymes that kill the most powerful antibiotics used against life-threatening, multidrug-resistant bacterial infections – coupled with the introduction of a bacterial clone that spread between patients created the “perfect storm” that led to today’s antibiotic resistance epidemic in Colombia. Results of this study, led by investigators from The University of Texas Health Science Center at Houston (UTHealth), Universidad El Bosque and Case Western Reserve University, have been published in the Journal of Infectious Diseases.

Senior author Cesar Arias, M.D., Ph.D., professor and director of the Center for Antimicrobial Resistance and Microbial Genomics at McGovern Medical School at UTHealth, and his fellow researchers found that the epidemic began with what he described as a “promiscuous” carbapenemase gene that spread horizontally across a particular type of Gram-negative bacteria. The gene produced an enzyme that broke down powerful carbapenems, the “last-resort” reliable antibiotic drugs used to treat Gram-negative bacteria. The origin of the gene is still unknown, but it spread rapidly from patient to patient throughout the country.

Researchers sequenced the genomes of 133 samples of Klebsiella pneumoniae, a bacterium that can cause serious illness including bloodstream infections, wound infections, pneumonia and meningitis. The samples, recovered from 24 hospitals across Colombia, were collected between 2002 and 2014, before and after the emergence of carbapenem resistance in these organisms. Colombia has a high density of antibiotic-resistant organisms compared to other countries and like many others, is struggling to combat an epidemic.

To exacerbate the crisis, shortly after the spread of the gene, a bacterial clone carrying a similar antibiotic-killing gene was introduced into the country, most likely via an infected patient who traveled from the U.S., where the clone was already present. This bacterial lineage carried multiple antibiotic resistance determinants and quickly spread through person-to-person contact, as happened before in other countries.

“With the availability of these data, we could pinpoint the major drivers of the epidemic: when the first strain was identified, how it was disseminated and how it reached this level of epidemic,” said Arias, who is also director of the Center for Infectious Diseases at UTHealth School of Public Health.

The approach used in this paper could help Colombia and other countries working to combat antibiotic resistance.

“If you have a bacteria passing from one patient to another, you can enact very strict infection control measures to stop an epidemic,” said Arias. “But how do you stop a gene passing from bacteria to bacteria if you can’t see it? The dissemination of the gene was fueled by the overuse of antibiotics because it could not easily be treated.”

According to Arias, this approach could potentially help researchers and health care providers target specific clinical settings where the gene is being disseminated versus where the clone is and enact either infection control or antibiotic stewardship measures.

“By tracking the molecular epidemiology of an epidemic like this, Laura Rojas and the team uncovered important lessons that will help us combat other resistant bacteria,” said Robert A. Bonomo, M.D., coauthor on the paper and professor of medicine at the Cleveland VA Medical Center and Case Western Reserve University School of Medicine. Laura Rojas was first author on the paper and is a graduate student at Case Western Reserve University School of Medicine.

Researchers from Case Western Reserve University; Louis Stokes Cleveland VA Medical Center; Jackson Laboratory for Genomic Medicine; International Center for Medical Research and Training in Cali, Colombia; International Center for Microbial Genomics Universidad El Bosque in Bogotá, Colombia; American Museum of Natural History; University of Pennsylvania and Children’s Hospital of Philadelphia were coauthors on the paper.

The research was funded by the National Institutes of Health’s National Institute of Allergy and Infectious Diseases grants K24-AI114818, R01-AI093749, R21-AI114961 and R21/R33-AI121519 (awarded to Arias) and grants R21AI114508, R01AI100560, R01AI063517 and R01AI072219 (awarded to Bonomo). Funds and facilities from the Cleveland Department of Veterans Affairs, VISN 10 Geriatric Research Education and Clinical Center and the Veterans Affairs Office Research and Development (1I01BX001974 to Bonomo) also supported the work.

Tags | Research



Social Posts

profile_image

Veterans Affairs

@DeptVetAffairs

Subscribe to Borne the Battle in iTunes and have these powerful Veteran stories delivered straight to your device e… https://t.co/MFb2CmjQ2k

16 mins ago
profile_image

UniversityofHouston

@UHouston

Wanna MEAT🍖 for lunch? NBA Hall of Famer & UH alumnus Clyde ‘The Glide' Drexler brings new BBQ restaurant to SC Sa… https://t.co/CCJALbAJLM

24 mins ago
profile_image

Memorial Hermann

@memorialhermann

Memorial Hermann Cypress Hospital & @CyFairISD are promoting physical activity in elementary school classrooms thro… https://t.co/hx4aB6hiYp

35 mins ago
profile_image

TAMU Health Sciences

@TAMHSC

#Conjuctivitis (#PinkEye) is one of the most common eye infections. Here's what you need to know to keep it at bay: https://t.co/J4nuFBidUT

43 mins ago
profile_image

Rice University

@RiceUniversity

Rice researchers attended the University Innovation & Entrepreneurship Showcase to demonstrate SNOWater, a solar wa… https://t.co/HsexnxBWVp

46 mins ago
profile_image

BCMHouston

@bcmhouston

Powering through pain is often seen as a sign of strength, but when do we reach our limit? @BCMEthics’ Julia Jung e… https://t.co/R4lEKkCljU

59 mins ago
profile_image

Baylor College of Medicine

BaylorCollegeOfMedicine

Powering through pain is often seen as a sign of strength, but when do we reach our limit? Baylor College of Medicine Center for Medical Ethics and Health Polic...

1 hour ago
profile_image

BCMHouston

@bcmhouston

RT @VEWotring: Surgery in space? So excited about today's Science Friday! George Pantalos will discuss his new project funded by Translati…

1 hour ago
profile_image

BCMHouston

@bcmhouston

RT @BCMHouston_News: Uptick in #mumps cases prompts 2 Texas prisons to close to visitors, but @bcmhouston infectious disease expert says ou…

1 hour ago
profile_image

Rice University

@RiceUniversity

https://t.co/DPFPzSD1bv

1 hour ago
profile_image

CHI St Luke's Health

@CHI_StLukes

Dr. Jeremiah Johnson, endovascular neurosurgeon at Baylor St. Luke’s Certified Comprehensive Stroke Center: “This [… https://t.co/vJLqRUYehS

1 hour ago
profile_image

TAMU Health Sciences

@TAMHSC

An associate professor from @TAMU_SPH represented @TAMU at the inaugural University Innovation and Entrepreneurship… https://t.co/nUw5MbpsCI

1 hour ago
profile_image

Veterans Affairs

@DeptVetAffairs

ICYMI: @USCG #VeteranOfTheDay Alexander G. Thompson Jr. served aboard the USS Ingham from during #WWII… https://t.co/15uRIQCNld

1 hour ago
profile_image

UTHealth

@UTHealth

RT @SallyMacFox26: Deep laceration thanks to @UTHealth clinical nurse educator, Kennessa, who is transforming dozens of students into victi…

1 hour ago
profile_image

TexasHeartInstitute

@Texas_Heart

Dog owners may have decreased risk of heart disease and mortality, new study suggests. https://t.co/8pwNcDTmgO

2 hours ago