Howard Gendelman, MD: Elimination of HIV-1 Infection in Humanized Mice
Houston Methodist Research Institute presents:
Howard Gendelman, MD
Margaret R. Larson Professor of Internal Medicine and Infectious Diseases Chair
Department of Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Treatment with long acting slow effective release antiretroviral therapy (LASER ART) rilpivirine, myristoylated dolutegravir, lamivudine and abacavir followed by CRISPR-Cas9 proviral DNA excision led to viral eradication in HIV-1 infected humanized mice. Ultrasensitive nested and digital droplet PCR and RNA scope assays failed to detect HIV-1 in blood, spleen, lung, kidney, liver, gut-associated lymphoid tissue and brain in twenty-nine percent of infected animals treated with the dual regimen.
Excision of proviral DNA fragments spanning the long terminal repeat (LTR) and the Gag gene were identified without detectable off target effects. The absence of viral rebound following cessation of ART with no progeny virus recovery served to verify HIV-1 eradication. In contrast, HIV-1 was readily detected in all infected animals treated with LASER ART or CRISPR-Cas9 alone. In this lecture, learn about how Howard E. Gendelman, MD, and his team have used sequential application of LASER ART and CRISPR-Cas9 therapies in HIV-1 infected humanized mice, to provide the proof of concept for viral sterilization.
For lecture abstract and location information, visit: events.houstonmethodist.org/howard-gendelman
Houston Methodist Research Institute
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Houston, Texas 77030
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