Ernst-Ludwig Florin: Activity Microscopy
The Center for Theoretical Biological Physics PRESENTS Seminar Speaker:
Center for Nonlinear Dynamics
University of Texas at Austin
Tuesday, October 31, 2017
12:30 – 1:30 PM
BRC, 10th Floor, Room 1060 A/B
Abstract: Cells migrating through the extracellular matrix probe and remodel individual filaments around them. The quantification of such cell-matrix interactions requires a novel type of microscopy that can visualize local network properties, and the network’s response to external forces with single filament resolution. This is a challenging task, because filaments, such as collagen fibrils, can be relatively stiff and under high tension, resulting in small – but important – filament fluctuations.
Here we introduce Activity Microscopy, a novel method based on high-bandwidth position detection. The local network architecture is extracted from the three-dimensional detector response. Transverse fluctuations are recorded along each filament with subnanometer precision and megahertz bandwidth. Activity Microscopy images show the network’s architecture overlaid with the magnitude of filament fluctuations which can be linked to the forces acting on the individual filament. Activity microscopy is expected to quantify cell-matrix interaction and to monitor mechanical cell-cell communication.
Bio: Ernst-Ludwig Florin earned his Ph.D. in Physics 1995 from the Technical University Munich, Germany. Dr. Florin is an Associate Professor in the Center for Nonlinear Dynamics and Department of Physics with a specialization in Biophysics at the University of Texas at Austin. He is interested in how physics can help to understand biological systems but also in how biological systems can be used to gain new insight into physics. He studies biological systems from the single molecule level to the population level. The development of new types of microscopy for biological research is a major part of his research effort.
An Official Seminar of the Ph.D. Program in Systems, Synthetic and Physical Biology at Rice University
For more information, visit: https://events.rice.edu/#!view/event/date/20171031/event_id/1030
BioScience Research Collaborative (BRC), 10th Floor, Room 1060 A/B
6500 Main St, Houston, TX 77030