Light is helping Rice University scientists control both the infectivity of viruses and gene delivery to the nuclei of target cells.
The researchers have developed a method to use two shades of red to control the level and spatial distribution of gene expression in cells via an engineered virus.
Although viruses have evolved to deliver genes into host cells, they still face difficulties getting their payloads from the cytoplasm into a cell’s nucleus, where gene expression occurs. The Rice labs of bioengineers Junghae Suh and Jeffrey Tabor have successfully found a way to overcome this critical hurdle.
The team’s research appears this week in the American Chemical Society journal ACS Nano.
The result from labs at Rice’s BioScience Research Collaborative combines Suh’s interest in designing viruses to deliver genes to target cells with Tabor’s skills in optogenetics, in which light-responsive proteins can be used to control biological behavior.
They built custom adeno-associated virus (AAV) vectors by incorporating proteins that naturally come together when exposed to red light (650-nanometer wavelengths) and break apart when exposed to far red (750-nanometer wavelengths). These naturally light-responsive proteins help the viral capsids – the hard shells that contain genetic payloads –enter the host cell nuclei.
AAV has been the vector of choice for gene editing by way of CRISPR/Cas9, a technique that shows promise for curing some genetic diseases, but cell nuclei present a problem, Suh said.
“Viruses in general are relatively efficient at delivering genes into cells, but they still experience great limiting barriers,” she said. “If you add these viruses to cells, most of them seem to hang out outside of the nucleus, and only a small fraction make their way inside, which is the goal.”
She said the team drew upon the Tabor lab’s expertise in optogenetics to increase the AAVs’ efficiency. “Jeff works with many different types of light-responsive proteins. The particular pair we decided upon was first identified in plants.
“Light is really nice because you can apply it externally and you can control many aspects: at what areas the light is exposed, the duration of exposure, the intensity of the light and, of course, its wavelength,” she said.
The protein pair comprises phytochrome B and its binding partner phytochrome interacting factor 6 (PIF6), both found in thale cress. The researchers generated host cells that express phytochrome B tagged with a nuclear localization sequence, a small peptide known to help shuttle proteins into the nucleus more effectively. The smaller PIF6 was then attached to the outside surface of the virus capsid.
“When the viruses get internalized into a host cell, they accumulate around the nucleus naturally,” Suh said. “Under nonactivated conditions, most of the viruses are stuck there. But when we shine activating red light on the cells, these two plant proteins dimerize — they come together — and because of the nuclear localization tag on the phytochrome B, the virus is dragged into the nucleus.”
This is the first time optogenetic proteins have been used to control the infectivity of viruses, she said, adding, “We didn’t think it would work as well as it did.”
Lead author Eric Gomez, a graduate student in Suh’s lab, and co-author Karl Gerhardt, a graduate student in Tabor’s lab, demonstrated the technique by activating patterns of AAV-infected cells in petri dishes by shining red light at them through masks.
Suh said the platform may be used in the future to control what cells and tissues express a gene and at what level. The strategy could also find use in tissue-engineering applications like bioscaffolds for implantation. “Here, you might want to coax stem cells to develop into the right cells in some kind of spatially coordinated fashion,” she said.
Use in the body is further off, because externally applied red light only goes so far through tissue to reach targets. “Ultimately, it’s going to depend on the device you use to deliver the light,” she said. “But we imagine cases where you could use a catheter to go to the spot where you want the viruses to be much more infectious.”
Suh said the Rice labs are working to make the next generation of capsids both more effective and more deliverable. “This is just the first step,” she said. “We have a lot of questions, like ‘Can we really do this in vivo?’ kind of questions.
“There have been a handful of papers trying to make the virus-infectious process responsive to light, but they were before the era of optogenetics,” Suh said. “They were using other types of synthetic molecules to make viruses light-responsive.
“It seems using what Mother Nature has already provided in the form of light-responsive proteins works really well for us.”
Rice alumnus Justin Judd, now a postdoctoral researcher at the University of California, San Francisco, is co-author of the paper. Suh is an associate professor of bioengineering. Tabor is an assistant professor of bioengineering.
A National Academy of Sciences Ford Foundation Fellowship to Gomez supported the research.
Good luck Houston Dash on tomorrow’s Opening Day! #DashOn #LeadingMedicine
Good luck @HoustonDash on tomorrow’s Opening Day! #DashOn #LeadingMedicine https://t.co/XMu3UQ1C73
TAMU Health Sciences@TAMHSC
#3DPrinting #technology may revolutionize the #pharmaceutical industry in the very near-future: https://t.co/i4Op8Eevce #TAMHSC
Expanding Traumatic Brain Injury treatment options for Veterans https://t.co/43peGDiznA via @VeteransHealth on #VAntagePoint
MD Anderson Cancer Center@MDAndersonNews
Mom’s advice on cancer during pregnancy: “Keep fighting, and don’t ever give up.“ #CancerMoonShot #endcancer https://t.co/vhxqcjALFI https://t.co/1y51kCCy2l
Today’s #VeteranOfTheDay is @USArmy Veteran Daniel J. Kerrigan. https://t.co/hojAQtCA6n
U.S. Department of Veterans AffairsVeteransAffairs
Today’s #VeteranOfTheDay is Army Veteran Daniel J. Kerrigan. Daniel served during Operation Enduring Freedom during 2004-2005. While living in Kalamazoo, Michigan, Daniel desired to serve his country in the United States Army. After initial trouble passing the military’s standard hearing test, Daniel enlisted in the Army on Oct. 25, 1990, as the military occupation specialty of 11X at 27-years-old. Daniel excelled in boot camp and infantry school. In July of 2004, Daniel deployed to the Kandahar province, Afghanistan, to participate in Operation Enduring Freedom. Attached to Charlie Company of the 25th Infantry division, he and his partner, Major Robert Doshee, were tasked to mentor and train an Afghani unit and their leaders. During his combat deployment in Afghanistan, he was awarded the Bronze Star with valor for saving the life of his friend, Steve DeYoung. Along with the Bronze Star, Daniel also received the Afghanistan Campaign medal and a Purple Heart. Daniel left active duty in 2007. Thank you for your service, Daniel!
RT @UTPhysicians: https://t.co/3NsWuRAHDF
RT @MDA_UTHGrad: Volunteers with Faithful Paws brought five therapy animals (4 dogs & a cat) to visit with Graduate School students in the…
RT @UTPhysicians: Thank you to everyone who came out today to celebrate the grand opening of UT Physicians - Jensen! https://t.co/tbbWOrPmtM
Hundreds of volunteers helping to clean up Chalmette @VANatCemeteries https://t.co/zudKD1DYBu via @WGNOtv
MD Anderson Cancer Center@MDAndersonNews
Survivor of multiple cancers @LainieMSchultz has advice for newly diagnosed patients: ”You’ll get through it.“ #endcancer https://t.co/rKYAmH20nj
We've all nicked ourselves shaving, but why do certain times cause you to bleed more than others? Dr. Iberia Sosa explains: https://t.co/vgmnux8Ofa
Baylor College of MedicineBaylorCollegeOfMedicine
Missed Match Day last week? Check out our recap. #Match2018 #MatchBCM
4.3, 6-8 p.m.: @MethodistHosp Willowbrook Hospital free informative session about surgical options for the next step toward achieving your weight loss goals https://t.co/TdgYrFuxGN https://t.co/vAAuaEVAHZ